Alien Chromosome Serves as a Novel Platform for Multiple Gene Expression in <i>Kluyveromyces marxianus</i>.

ABSTRACT: Kluyveromyces marxianus is an emerging yeast cell host for diverse products, but multiple-gene expression in K. marxianus faces challenges due to limited current knowledge of cis-regulatory elements and insertion loci. Our previous study transferred an alien Saccharomyces cerevisiae chromosome I (R1) into K. marxianus, resulting in the creation of the monochromosomal hybrid yeast KS-R1. All R1 genes were actively transcribed, providing a series of loci with varying transcriptional activities. Here, we explore the use of R1 as a novel platform for stable, multi-gene integration and expression. By deleting three essential K. marxianus genes while complementing their functions with orthologs on R1, we achieved stable propagation of R1 in the absence o

SUBMITTER: Lyu Y 

PROVIDER: S-EPMC11946330 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature