Effects of Crude Shea Butters and Their Polar Extracts on Singlet Oxygen Quenching and Against Rose Bengal-Induced HaCaT Cell Phototoxicity.

ABSTRACT: Shea butter (SB) is a raw material fat obtained from Vitellaria paradoxa C.F. Gaertn kernels. We investigated the direct and indirect protective effects of 10 traditional and industrial SBs and their polar extracts on cell-free systems using ABTS and DPPH radical scavenging assays as well as on singlet oxygen (¹O₂) produced by Rose Bengal (RB) photosensitization. Their effects against RB-induced HaCaT cell phototoxicity were also explored. A spectrophotometric assay and HPLC were performed to quantify and identify phenolic content, which was between 14.16 and 82.99 ppm pyrogallol equivalent. These variations could be due to the SB origin and extraction process. These polar fractions exhibited moderate DPPH and strong ABTS radical-scavenging activity. By applyin

SUBMITTER: Goumbri BWF 

PROVIDER: S-EPMC11946392 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature