Diagnostic Performance of Rapid Antigen Tests to Detect Equine Rotavirus A.

ABSTRACT: This study describes a comparison of the detection of rotavirus in clinical samples from foals using two commercially available rapid antigen detection (RAD) kits, with the detection of rotavirus nucleic acid via a laboratory-based, in-house, real-time reverse transcription polymerase chain reaction (RT-PCR) assay. One hundred and forty freeze-thawed samples (70 that were RT-PCR-positive and 70 that were RT-PCR-negative on original tests) submitted to the diagnostic laboratory over a seven-year period were tested in addition to 123 fresh samples (15 RT-PCR-positive and 108 RT-PCR-negative) submitted over a four- month period in 2024. The analyst performing the RAD tests was blinded to the RT-PCR result as were the two individuals who read the results. Samples with discordant results were r

SUBMITTER: Cullinane A 

PROVIDER: S-EPMC11946396 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature