Detection and In Vivo Validation of <i>Dichorhavirus</i> e-Probes in Meta-Transcriptomic Data via Microbe Finder (MiFi^®) Discovers a Novel Host and a Possible New Strain of Orchid Fleck Virus.

ABSTRACT: Dichorhavirus is a recently accepted plant virus genus within the family Rhabdoviridae. Species assigned to the genus consist of bi-segmented, negative sense, single-stranded RNA viruses and are transmitted by Brevipalpus spp. Currently, there are five recognized species and two unclassified members in the genus Dichorhavirus. Four out of seven-orchid fleck virus (OFV), citrus leprosis virus N, citrus chlorotic spot virus, and citrus bright spot virus-can infect citrus and produce leprosis disease-like symptoms. The E-probe Diagnostic for Nucleic Acid Analysis (EDNA) was developed to reduce computational effort and then integrated within Microbe-Finder (MiFi^®) online platform to design and evaluate e-probes in raw High Throughput Sequencing (HTS) data

SUBMITTER: Roy A 

PROVIDER: S-EPMC11946451 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature