Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803.

ABSTRACT: One potential approach to improve the productivity of cyanobacteria and microalgae is to enhance photosynthetic efficiency by introducing far-red absorbing pigment molecules (such as chlorophylls f and d) into the photosynthetic apparatus to expand the range of photosynthetically active radiation. We have shown previously that expressing the ChlF subunit of Chroococcidiopsis thermalis PCC 7203 in the model cyanobacterium Synechocystis sp. PCC 6803 (Syn6803) is sufficient to drive the production of chlorophyll f (Chl f), but only to low levels (0.24% Chl f/Chl a). By using the strong P_cpc560 promoter and an N-terminal truncated derivative of ChlF, we have been able to increase the yield of Chl f in white light by over 30-fold to about 8.2% Chl f/Chl a, close to the level displaye

SUBMITTER: Qi M 

PROVIDER: S-EPMC11946780 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature