Structure and mechanism of the Zorya anti-phage defence system.

ABSTRACT: Zorya is a recently identified and widely distributed bacterial immune system that protects bacteria from viral (phage) infections. Three Zorya subtypes have been identified, each containing predicted membrane-embedded ZorA-ZorB (ZorAB) complexes paired with soluble subunits that differ among Zorya subtypes, notably ZorC and ZorD in type I Zorya systems^1,2. Here we investigate the molecular basis of Zorya defence using cryo-electron microscopy, mutagenesis, fluorescence microscopy, proteomics and functional studies. We present cryo-electron microscopy structures of ZorAB and show that it shares stoichiometry and features of other 5:2 inner membrane ion-driven rotary motors. The ZorA₅B₂ complex contains a dimeric ZorB peptidoglycan-binding domain and a penta

SUBMITTER: Hu H 

PROVIDER: S-EPMC11946911 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature