Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork.

ABSTRACT: Streptococcus suis is a major pathogen in pigs, causing diseases like meningitis, septicemia, and pneumonia, and has become a serious zoonosis in humans, particularly in countries with intensive swine production. Human infections have risen significantly, especially in Thailand, where serotype 2 is most commonly associated with disease in both pigs and humans. Traditional methods of identifying S. suis and its serotypes, such as bacterial culture, biochemical testing, and agglutination tests, face challenges due to variability and limited antisera availability, leading to the need for alternative approaches. In this study, we developed a novel RPA-NALFIA assay targeting the recN and cps2J genes of S. suis serotype 2. This method demonstrated accurate identification without cross-reactivity

SUBMITTER: Eiamphungporn W 

PROVIDER: S-EPMC11947096 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature