Cocaine perturbs neurodevelopment and increases neuroinflammation in a prenatal cerebral organoid model.

ABSTRACT: Prenatal exposure to cocaine causes abnormalities in foetal brain development, which are linked to later development of anxiety, depression and cognitive dysfunction. Previous studies in rodent models have indicated that prenatal cocaine exposure affects proliferation, differentiation and connectivity of neural cell types. Here, using cerebral organoids derived from the human iPSC cell line HPSI1213i-babk_2, we investigated cocaine-induced changes of the gene expression regulatory landscape at an early developmental time point, leveraging recent advances in single cell RNA-seq and single cell ATAC-seq. iPSC-cerebral organoids replicated well-established cocaine responses observed in vivo and provided additional information about the cell-type specific regulation of gene expression followin

SUBMITTER: Davis JL 

PROVIDER: S-EPMC11947122 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature