M⁶A modification regulates cell proliferation via reprogramming the balance between glycolysis and pentose phosphate pathway.

ABSTRACT: N6-methyladenosine (m⁶A) stands as the predominant modification in eukaryotic mRNA and is involved in various biological functions. Aberrant m⁶A has been implicated in abnormal cellular phenotypes, including defects in stem cell differentiation and tumorigenesis. However, the precise effects of m⁶A on cell proliferation and the underlining mechanism of metabolic gene regulation remain incompletely understood. Here, we established a cellular environment with low-m⁶A levels and observed a severe impairment of cell proliferation. Mechanistic studies revealed that the depletion of m⁶A on TIGAR mRNA led to increased expression, subsequently inhibiting glycolysis while promoting the pentose phosphate pathway (PPP). A genome-wide CRISPR-Cas9

SUBMITTER: Xi JF 

PROVIDER: S-EPMC11947274 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature