Tet2 and tet3 regulate cell fate specification and differentiation events during retinal development.

ABSTRACT: Tet family methylcytosine dioxygenases recognize and oxidize 5-methyl-cytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Previous work demonstrated the requirement for Tet and 5hmC during zebrafish retinogenesis. tet2^-/-;tet3^-/- mutants possessed defects in the formation of differentiated retinal neurons, but the mechanisms underlying these defects are unknown. Here, we leveraged scRNAseq technologies to better understand cell type-specific deficits and molecular signatures underlying the tet2^-/-;tet3^-/- retinal phenotype. Our results identified defects in tet2^-/-;tet3^-/- retinae that included delayed specification of several retinal cell types, reduced maturity across late-stage cones, expansions of immature subpopulations o

SUBMITTER: Heilman SA 

PROVIDER: S-EPMC11947307 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature