Pig jejunal single-cell RNA landscapes revealing breed-specific immunology differentiation at various domestication stages.

ABSTRACT:

Background

Domestication of wild boars into local and intensive pig breeds has driven adaptive genomic changes, resulting in significant phenotypic differences in intestinal immune function. The intestine relies on diverse immune cells, but their evolutionary changes during domestication remain poorly understood at single-cell resolution.

Methods

We performed single-cell RNA sequencing (scRNA-seq) and marker gene analysis on jejunal tissues from wild boars, a Chinese local breed (Jinhua), and an intensive breed (Duroc). Then, we developed an immune cell evaluation system that includes immune scoring, gene identification, and cell communication analysis. Additionally, we mapped domestication-related clustering relationships, highlighting changes in gene expression and immune

SUBMITTER: Fu W 

PROVIDER: S-EPMC11947726 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature