Allosteric Probe Recognition-Induced Exponential Amplification Reaction for Label-Free Photosensitization Colorimetric Detection of Small Extracellular Vesicles.

ABSTRACT: The expression levels of small extracellular vesicles (sEVs) are acknowledged as highly promising diagnostic biomarkers for a variety of diseases, including chronic obstructive pulmonary disease and lung cancer. However, a convenient and sensitive system for on-site rapid detection of sEVs is still highly desired. We have created a portable and highly efficient photosensitization colorimetric platform for label-free, sensitive, and colorimetric sEV detection by using an allosteric probe to specifically identify the CD63 protein on the surface of sEVs, which triggers numerous signal cycles to generate color changes. Visual detection of sEVs could be accomplished in 60 min using this detection platform. It was noted that this detection platform was capable of effectively detecting the target

SUBMITTER: Wu C 

PROVIDER: S-EPMC11947822 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature