Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl-CoA Pathway at High Titer.

ABSTRACT: Malonic acid (MA) is a high-value-added chemical with significant applications in the polymers, pharmaceutical, and food industries. Microbial production of MA presents enzyme inefficiencies, competitive metabolic pathways, and dispersive carbon flux, which collectively limit its biosynthesis. Here, the non-conventional oleaginous yeast Yarrowia lipolytica is genetically engineered to enhance MA production. Initially, the malonyl-CoA pathway, comprising a malonyl-CoA hydrolase from Saccharomyces cerevisiae, is confirmed as the most efficient for MA production in Y. lipolytica. To further enhance MA production, two novel malonyl-CoA hydrolases exhibiting higher activity than the hydrolase from S. cerevisiae, are identified from Y. lipolytica and Fusarium oxysporum, respectively. The introdu

SUBMITTER: Yang Q 

PROVIDER: S-EPMC11947988 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature