Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria.

ABSTRACT: Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO₂ shell (MagMQD@Si⁺) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen. The supe

SUBMITTER: Li J 

PROVIDER: S-EPMC11948010 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature