High-Throughput Solid Phase Extraction for Targeted and Nontargeted Exposomics.

ABSTRACT: Characterizing the chemical exposome relies on advanced instrumentation including tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS), and nontargeted analysis (NTA) using high-resolution MS. However, proper sample pretreatment, balancing broad analyte coverage, method robustness, and throughput remain a major bottleneck in exposomics. Here, we developed a robust and scalable solid phase extraction (SPE) protocol for human urine and plasma and optimized it for a panel of 94 highly diverse environmental and food-related contaminants (LogP -0.7 to 6.8). Extraction recoveries (RE) and signal suppression and enhancement (SSE) were determined using targeted LC-MS/MS. Acceptable REs (60-140%) were achieved for >70% of analytes, and acceptable SSE values (60-140%) for 86% and 90%

SUBMITTER: Gu Y 

PROVIDER: S-EPMC11948174 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature