Studying Cationic Liposomes for Quick, Simple, and Effective Nucleic Acid Preconcentration and Isolation.

ABSTRACT: To ensure high quality of food and water, the identification of traces of pathogens is mandatory. Rapid nucleic acid-based tests shorten traditional detection times while maintaining low detection limits. Challenging is the loss of nucleic acids during necessary purification processes, since elution off solid surfaces is not efficient. We therefore propose the development of a vanishing surface strategy in which cationic liposomes efficiently capture nucleic acids. A lipoplex is formed that can be easily centrifuged down and washed if needed. Adding the lipoplex to detergent solutions or nucleic acid amplification reactions dissolves the liposomes, releasing 100% of the nucleic acid into the reaction. After initial protocol optimization, it was applied to isolate DNA from Escherichia co

SUBMITTER: Gruenberger R 

PROVIDER: S-EPMC11948181 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature