Highly multiplexed imaging reveals prognostic immune and stromal spatial biomarkers in breast cancer.

ABSTRACT: Spatial profiling of tissues promises to elucidate tumor-microenvironment interactions and generate prognostic and predictive biomarkers. We analyzed single-cell spatial data from 3 multiplex imaging technologies: cyclic immunofluorescence (CycIF) data we generated from 102 patients with breast cancer with clinical follow-up as well as publicly available mass cytometry and multiplex ion-beam imaging datasets. Similar single-cell phenotyping results across imaging platforms enabled combined analysis of epithelial phenotypes to delineate prognostic subtypes among patients who are estrogen-receptor+ (ER+). We utilized discovery and validation cohorts to identify biomarkers with prognostic value. Increased lymphocyte infiltration was independently associated with longer survival in triple-nega

SUBMITTER: Eng JR 

PROVIDER: S-EPMC11948582 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature