Amino acid metabolism pathways as key regulators of nitrogen distribution in tobacco: insights from transcriptome and WGCNA analyses.

ABSTRACT:

Background and aim

Nitrogen (N) is crucial for plant growth and is distributed across various N morphologies within plant organs. However, the mechanisms controlling the distribution of these N morphologies are not fully understood. This study investigated key amino acid (AA) biosynthesis pathways regulating N distribution and their impact on plant physiology and growth.

Methods

We examined N distribution in the leaves, stems, and roots of two tobacco cultivars (Hongda and K326) under different N treatments at 75, and 100 days after transplanting (DAT). Transcriptome analysis was performed at 75 and 100 DAT to explore N distribution and AA metabolism pathways. Weighted gene co-expression network analysis (WGCNA) identified pathways regulating N distribution, and the Mantel t

SUBMITTER: Li S 

PROVIDER: S-EPMC11948770 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature