Effect of low-level laser on proliferation, angiogenic and dentinogenic differentiation of human dental pulp stem cells.

ABSTRACT:

Background

The aim was to evaluate the effect of single and double doses of low-level laser irradiation on proliferation of human dental pulp stem cells (DPSC) and expression of vascular endothelial growth factor (VEGF) and dentine sialoprotein (DSP).

Methods

In this experimental in vitro study, after confirming the stemness of DPSCs, the cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) for MTT assay and VEGF-ELISA and osteogenic medium for DSP-ELISA. The wells containing DPSCs were divided into three main groups and 9 subgroups (n = 7). In groups with single low-level laser, 660-nm diode laser was irradiated at 100 mW and 3 J/cm² energy density for 15 s. In groups with double doses of low-level laser the second identical irradiation was after 48 h.

SUBMITTER: Rezaei F 

PROVIDER: S-EPMC11948823 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature