Exploring the synergy between fungal CE15 glucuronoyl esterases and xylanases for lignocellulose saccharification.

ABSTRACT:

Background

Lignin-carbohydrate complexes in lignocellulosic biomass act as a barrier to its biodegradation and biotechnological exploitation. Enzymatic dissociation between lignin and hemicellulose is a key process that allows the efficient bioconversion of both polymers. Glucuronoyl esterases of the Carbohydrate Esterase 15 family target the ester linkages between the glucuronic acid of xylan and lignin moieties, assisting enzymatic biodegradation of lignocellulose.

Results

In this study, two CE15 glucuronoyl esterases from the white-rot fungi Artolenzites elegans and Trametes ljubarskyi were heterologously expressed in Pichia pastoris and biochemically characterized on the model substrate D-glucuronic acid ester with cinnamyl alcohol and a variety of pretreated lignocellul

SUBMITTER: Pentari C 

PROVIDER: S-EPMC11948903 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature