Diagnosis of celiac disease on a gluten-free diet: a multicenter prospective quasi-experimental clinical study.

ABSTRACT:

Background

Diagnosing celiac disease (CD) in individuals adhering to a gluten-free diet (GFD) presents significant challenges. Current guidelines recommend a gluten challenge (GC) lasting at least 6-8 weeks, which has several limitations. Our aim was to compare four approaches previously proposed for diagnosing CD on a GFD: IL-2 serum levels, gut-homing CD8⁺ T cells, % TCRγδ⁺ intraepithelial lymphocytes (IELs), and UBE2L3 gene expression. Additionally, we evaluated the CD8⁺ T-cell-based method with a 3-day GC against the standard GC protocol.

Methods

We conducted a multicenter prospective quasi-experimental clinical study. Two subsets of individuals were considered: (1) 20 patients with CD previously diagnosed and 15 non-CD controls, to evalu

SUBMITTER: Gomez-Aguililla S 

PROVIDER: S-EPMC11948932 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature