Genome-wide characterization of FAD gene family in Xanthoceras sorbifolium Bunge and germplasm assessment.

ABSTRACT: Fatty acid desaturases (FADs) play a pivotal role in the accumulation of oils in plant seeds. To elucidate the role of FADs in oil accumulation in the seeds of Xanthoceras sorbifolium, this study employed bioinformatics analysis methods to identify and analyze the FAD gene family. A total of 25 XsFAD genes were unevenly distributed across 11 chromosomes. Systematic phylogenetic analysis revealed that the XsFAD gene family is divided into three branches, with each branch exhibiting similar gene structures. The regulatory elements within the XsFAD gene promoter indicate that XsFAD genes are regulated by multiple factors. Quantitative real-time polymerase chain reaction (qRT-PCR) validation revealed a positive correlation between the expression levels of XsFAD genes and the oil content of X.

SUBMITTER: Xing Q 

PROVIDER: S-EPMC11949342 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature