Molecular analysis of AmpC-producing Escherichia coli isolated from pediatric patients.

ABSTRACT:

Background

AmpC-mediated cephalosporin resistance occurs in 1.0% to 3.3% of Escherichia coli isolates due to the production of either plasmid-mediated AmpC (p-AmpC) or chromosomal AmpC (c-AmpC). Data on the prevalence and molecular characteristics of AmpC-producing E. coli in pediatric patients are limited.

Methods

We analyzed E. coli clinical strains with resistance phenotype consistent with AmpC production isolated from patients at a pediatric hospital in Japan between 2015 and 2022. Sequence types, resistance genes, and relevant mutations were identified through whole genome sequencing. Promoter and attenuator regions of the chromosomal ampC gene were examined, and the presence of plasmid-mediated ampC genes was determined.

Results

Among 2081 E. coli strains, 80 (3

SUBMITTER: Ogawa E 

PROVIDER: S-EPMC11949381 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature