The antiprotease Spink7 promotes inflammation resolution by modulating multiple proteases activities during wound healing.

ABSTRACT:

Background

Effective control of inflammation is crucial for the healing of cutaneous wounds, but the molecular mechanisms governing inflammation resolution during wound closure are still not yet clear. Here, we describe a homeostatic mechanism that facilitates the inflammation resolution by timely regulating the targeted proteases activities through antiprotease Spink7 (serine peptidase inhibitor, kazal type 7).

Methods

The expression pattern of Spink7 was investigated by quantitative RT-PCR, immunohistochemistry (IHC) and in situ hybridization. In both Spink7 knockdown and knockout models, quantitative comparisons were made between the healing rate of wounds and histopathological morphometric analysis. Microarrays, multiple chemokine assays, IHC, immunofluorescence, proteas

SUBMITTER: Zhao N 

PROVIDER: S-EPMC11949503 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature