Pyruvate kinase modulates the link between β-cell fructose metabolism and insulin secretion.

ABSTRACT: The intricate link between glucose metabolism, ATP production, and glucose-stimulated insulin secretion (GIIS) in pancreatic β-cells has been well established. However, the effects of other digestible monosaccharides on this mechanism remain unclear. This study examined the interaction between intracellular fructose metabolism and GIIS using MIN6-K8 β-cell lines and mouse pancreatic islets. Fructose at millimolar concentrations potentiated insulin secretion in the presence of stimulatory levels (8.8 mM) of glucose. This potentiation was dependent on sweet taste receptor-activated phospholipase Cβ2 (PLCβ2) signaling. Concurrently, metabolic tracing using ¹³C-labeled fructose and glucose in conjunction with biochemical analyses demonstrated that fructose blunted the glucose-induce

SUBMITTER: Murao N 

PROVIDER: S-EPMC11950909 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature