SARS-CoV-2 serotyping based on spike antigenicity and its implications for host immune evasion.

ABSTRACT:

Background

As SARS-CoV-2 continues to spread and evolve, new variants/sub-variants emerge, raising concerns about vaccine-induced immune escape. Here, we conducted a systematic analysis of the serology and immunogenicity of major circulating variants/sub-variants of SARS-CoV-2 since the outbreak.

Methods

We expressed and purified trimeric S proteins from 21 SARS-CoV-2 variants, with SARS-CoV included as an outgroup. Mice were immunized, and the resulting antisera were tested for binding antibodies after the third dose injection, and for neutralizing antibodies (NAbs) after both the second and third doses. Using pseudovirus neutralization assays, we evaluated cross-neutralization among major circulating variants. By integrating serological classification, antigenic mapping, a

SUBMITTER: Ruan W 

PROVIDER: S-EPMC11951033 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature