Low-biomass pyruvate production with engineered Vibrio natriegens is accompanied by parapyruvate formation.

ABSTRACT:

Background

Pyruvate is a precursor for various compounds in the chemical, drug, and food industries and is therefore an attractive target molecule for microbial production processes. The fast-growing bacterium Vibrio natriegens excels with its specific substrate uptake rate as an unconventional chassis for industrial biotechnology. Here, we aim to exploit the traits of V. natriegens for pyruvate production in fermentations with low biomass concentrations.

Results

We inactivated the pyruvate dehydrogenase complex in V. natriegens Δvnp12, which harbors deletions of the prophage regions vnp12. The resulting strain V. natriegens Δvnp12 ΔaceE was unable to grow in minimal medium with glucose unless supplemented with acetate. In shaking flasks, the strain showed a growth rate of 1

SUBMITTER: Hadrich M 

PROVIDER: S-EPMC11951559 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature