Development of a monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of antibodies against duck adenovirus 3.

ABSTRACT:

Background

Duck adenovirus 3 (DAdV-3), the emerging pathogen of a disease characterized by swelling and hemorrhage in liver and kidney, is widely distributed in China, resulting in serious economic losses to the duck farms. However, no vaccine is commercially available for the prevention of the disease. Thus, a precise and reliable diagnosis is of great value for the implementation of control measure to limit the spread of DAdV-3 infection. In this study, a competitive enzyme-linked immunosorbent assay (c-ELISA) was developed for detection of antibodies against DAdV-3.

Results

In the c-ELISA, a monoclonal antibody (mAb) 3D9, which was specific to Fiber-2 protein of DAdV-3, was labeled with horseradish peroxidase (HRP) and then used as secondary antibody. The cut-off value of

SUBMITTER: Lin Y 

PROVIDER: S-EPMC11951781 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature