Quantification of cellular phototoxicity of organelle stains by the dynamics of microtubule polymerization.

ABSTRACT: Being able to quantify the phototoxicity of dyes and drugs in live cells allows biologists to better understand cell responses to exogenous stimuli during imaging. This capability further helps to design fluorescent labels with lower phototoxicity and drugs with better efficacy. Conventional ways to evaluate cellular phototoxicity rely on late-stage measurements of individual or different populations of cells. Here, we developed a quantitative method using intracellular microtubule polymerization as a rapid and sensitive marker to quantify early-stage phototoxicity. Implementing this method, we assessed the photosensitization induced by organelle dyes illuminated with different excitation wavelengths. Notably, fluorescent markers targeting mitochondria, nuclei, and endoplasmic reticulum ex

SUBMITTER: Mahapatra S 

PROVIDER: S-EPMC11951871 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature