Development of a Novel mRNA Vaccine Against <i>Shigella</i> Pathotypes Causing Widespread Shigellosis Endemic: An In-Silico Immunoinformatic Approach.

ABSTRACT: Shigellosis remains a major global health concern, particularly in regions with poor sanitation and limited access to clean water. This study used immunoinformatics and reverse vaccinology to design a potential mRNA vaccine targeting Shigella pathotypes out of 4071 proteins from Shigella sonnei str. Ss046, 4 key antigenic candidates were identified: putative outer membrane protein (Q3YZL0), PapC-like porin protein (Q3YZM5), putative fimbrial-like protein (Q3Z3I2), and lipopolysaccharide (LPS)-assembly protein LptD (Q3Z5V5), ensuring broad pathotype coverage. A multitope vaccine was designed incorporating cytotoxic T lymphocyte, helper T lymphocyte, and B-cell epitopes, linked with suitable linkers and adjuvants to enhance immunogenicity. Computational analyses predicted vacci

SUBMITTER: Razzak A 

PROVIDER: S-EPMC11951904 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature