Impact of smoking habit on the subgingival proteome in patients with periodontitis.

ABSTRACT:

Background

Few investigations evaluated smoking's impact on the periodontal proteome. Therefore, this study aimed to analyse the influence of tobacco on the overall periodontal proteome and the differential expression of gingival crevicular fluid (GCF) proteins using sequential window acquisition of all theoretical mass spectra (SWATH-MS).

Methods

GCF samples were collected from 40 periodontitis subjects (stages III-IV). These were separated based on smoking status into smokers (17), ex-smokers (10), and non-smokers (13). Samples were analysed using SWATH-MS, and proteins were identified using the UniProt human-specific database. Data are available via ProteomeXchange with the identifier PXD043474. Principal component analysis (PCA) was employed to examine the spectral mass

SUBMITTER: Blanco-Pintos T 

PROVIDER: S-EPMC11951952 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature