Birmingham-group IncP-1<i>α</i> plasmids revisited: RP4, RP1 and RK2 are identical and their remnants can be detected in environmental isolates.

ABSTRACT: RP4, RP1, RK2 and R68 were isolated from the multidrug-resistant bacterial wound isolates in 1969 in the Birmingham Accident Hospital, Birmingham, England, and collectively called Birmingham-group IncP-1α plasmids. These plasmids have been widely used as models to study different aspects of plasmid biology, develop genetic delivery systems and design plasmid vectors. Early studies showed that these plasmids conferred the same antibiotic resistance profile, had a similar size and were undistinguishable from each other using DNA heteroduplex electron microscopy and restriction endonuclease analyses. These observations have led to the widely held assumption that they are identical, although there has been no conclusive supporting evidence. In this work, we sequenced the plasmids RP1 an

SUBMITTER: Le VVH 

PROVIDER: S-EPMC11952213 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature