Functional profiling of synthetic camel milk-derived peptides with implication in glucose transport and diabetes.

ABSTRACT: We previously identified protein hydrolysates from camel milk (CM) targeting dipeptidyl peptidase IV (DPP-IV) and insulin receptor (IR) activity. In this study, we synthesized nine peptides (P1 to P9) derived from such CM hydrolysates and profiled their potential bioactivity in vitro and in silico. This aims to validate and determine if such synthetic and pure CM-derived peptides are bioactive on IR function and glucose uptake using pharmacological and functional approaches in human embryonic kidney (HEK293) and human hepato-carcinoma (HepG2) cells. Our bioluminescence resonance energy transfer (BRET) results showed a partial activity of most peptides on IR activity in HEK293 cells ranging from 13 ± 1% to 65 ± 4%, and their potency varies from 3.13 ± 1.72 μg/ml to 12.30 ± 5.66 μg/ml. Combi

SUBMITTER: Anwar I 

PROVIDER: S-EPMC11952234 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature