Two-Stage CD8⁺ CAR T-Cell Differentiation in Patients with Large B-Cell Lymphoma.

ABSTRACT: Chimeric antigen receptor (CAR) T-cell therapy has expanded therapeutic options for patients with diffuse large B-cell lymphoma (DLBCL). However, progress in improving clinical outcomes has been limited by an incomplete understanding of CAR T-cell differentiation in patients. To comprehensively investigate CAR T-cell differentiation in vivo, we performed single-cell, multimodal, and longitudinal analyses of CD28-costimulated CAR T cells from infusion product and peripheral blood (day 8-28) of patients with DLBCL who were successfully treated with axicabtagene ciloleucel. Here, we show that CD8⁺ CAR T cells undergo two distinct waves of clonal expansion. The first wave is dominated by CAR T cells with an exhausted-like effector memory phenotype during the peak expansion period (d

SUBMITTER: Cao G 

PROVIDER: S-EPMC11952315 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature