Axially decoupled photo-stimulation and two photon readout (ADePT) for mapping functional connectivity of neural circuits.

ABSTRACT: All optical physiology in vivo provides a conduit for investigating the function of neural circuits in 3-D. Here, we report a new strategy for flexible, axially-decoupled photo-stimulation and two photon readout (ADePT) of neuronal activity. To achieve axially-contained widefield optogenetic patterned stimulation, we couple a digital micro-mirror device illuminated by a solid-state laser with a motorized holographic diffuser. In parallel, we use multiphoton imaging of neural activity across different z-planes. We use ADePT to analyze the excitatory and inhibitory functional connectivity of the mouse early olfactory system. Specifically, we control the activity of individual input glomeruli on the olfactory bulb surface, and map the ensuing responses of output mitral and tufted cell bodies

SUBMITTER: Koh M 

PROVIDER: S-EPMC11952351 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature