Factors affecting CRISPR-Cas defense against antibiotic resistance plasmids harbored by <i>Enterococcus faecalis</i> laboratory model strains and clinical isolates.

ABSTRACT: Enterococcus faecalis is a Gram-positive bacterium and opportunistic pathogen that acquires resistance to a wide range of antibiotics by horizontal gene transfer (HGT). The rapid increase of multidrug-resistant (MDR) bacteria including MDR E. faecalis necessitates the development of alternative therapies and a deeper understanding of the factors that impact HGT. CRISPR-Cas systems provide sequence-specific defense against HGT. From previous studies, we know that E. faecalis CRISPR-Cas provides sequence-specific anti-plasmid defense during agar plate biofilm mating and in the murine intestine. Those studies were mainly conducted using laboratory model strains with a single, CRISPR-targeted plasmid in the donor. MDR E. faecalis typically possess multiple plasmids

SUBMITTER: Ratna TA 

PROVIDER: S-EPMC11952401 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature