Canonical Wnt Signaling Suppresses Brain Endothelial Cell Transcytosis to Maintain Blood-Brain Barrier Integrity.

ABSTRACT: Canonical Wnt signaling is essential for blood-brain barrier (BBB) development and maintenance. However, the subcellular mechanisms underlying this critical regulation have remained elusive. In this study, we use a physiological paradigm examining an early phase of acutely attenuated canonical Wnt signaling in adult brain endothelial cells (ECs) to investigate how the pathway regulates BBB integrity. Following canonical Wnt signaling attenuation via EC-specific knockout of β-catenin, we find that there is increased transcytosis in brain ECs, including a striking diversity of morphologically distinct vesicles, indicating multiple pathways are involved. In addition, we find that although the molecular composition of tight junctions (TJs) is altered following canonical Wnt signaling attenuati

SUBMITTER: du Maine X 

PROVIDER: S-EPMC11952413 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature