"The <i>B. subtilis</i> replicative polymerases bind the sliding clamp with different strengths to tune replication processivity and fidelity".

ABSTRACT: Ring-shaped sliding clamp proteins are essential components of the replication machinery, the replisome, across all domains of life. In bacteria, DNA polymerases bind the sliding clamp, DnaN, through conserved short peptide sequences called clamp-binding motifs. Clamp binding increases the processivity and rate of DNA synthesis and is generally required for polymerase activity. The current understanding of clamp-polymerase interactions was elucidated in the model bacterium Escherichia coli, which has a single replicative polymerase, Pol III. However, many bacteria have two essential replicative polymerases, such as PolC and DnaE in Bacillus subtilis. PolC performs the bulk of DNA synthesis whereas the error-prone DnaE only synthesizes short stretches of DNA on the lagging str

SUBMITTER: O'Neal LG 

PROVIDER: S-EPMC11952451 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature