ER calcium stores contribute to glucose-induced Ca²⁺ waves and intercellular connectivity in mouse pancreatic islets.

ABSTRACT: Defective insulin secretion is a hallmark of diabetes mellitus. Glucose-induced Ca²⁺ oscillations are critical for the stimulation of insulin secretion, though the mechanisms through which these propagate across the islet are poorly understood. Here, we use beta cell-targeted GCaMP6f to explore the role of endoplasmic reticulum (ER) Ca²⁺ mobilization in response to submaximal (11mM) and hyperglycemic (25mM) glucose concentrations. Inhibition of inositol 1,4,5 trisphosphate (IP₃) receptors, and other ion channels, with 2-aminoethoxydiphenyl borate (2-APB) had minimal effects on the initial peak or intercellular connectivity provoked by 11mM glucose. However, 2-APB lowered subsequent glucose-induced cytosolic Ca²⁺ increases and connectivity at both

SUBMITTER: Delgadillo-Silva LF 

PROVIDER: S-EPMC11952499 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature