Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.

ABSTRACT: High-throughput screening and directed evolution using microfluidic picoreactors have produced high-activity enzymes. In this approach, a substrate is co-encapsulated with a candidate enzyme and individual picoreactors are sorted based on an activity reporter. While many approaches use water-in-oil droplets (single emulsions) for fluorescence-activated droplet sorting (FADS) on custom-fabricated microfluidic devices that require integrated optics and electronics, recent approaches have lowered the engineering barriers to adoption by using simple microfluidic droplet generators to produce water-in-oil-in-water droplets (double emulsion picoreactors, DEs) that can be sorted with commercial FACS (fluorescence-activated cell sorting). Despite the simplified engineering requirements, high varia

SUBMITTER: Yang Z 

PROVIDER: S-EPMC11952560 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature