Mesoscale molecular assembly is favored by the active, crowded cytoplasm.

ABSTRACT: The mesoscale organization of molecules into membraneless biomolecular condensates is emerging as a key mechanism of rapid spatiotemporal control in cells. Principles of biomolecular condensation have been revealed through in vitro reconstitution. However, intracellular environments are much more complex than test-tube environments: they are viscoelastic, highly crowded at the mesoscale, and are far from thermodynamic equilibrium due to the constant action of energy-consuming processes. We developed synDrops, a synthetic phase separation system, to study how the cellular environment affects condensate formation. Three key features enable physical analysis: synDrops are inducible, bioorthogonal, and have well-defined geometry. This design allows kinetic analysis of synDrop assembly a

SUBMITTER: Shu T 

PROVIDER: S-EPMC11952695 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature