CD147-CAR-NK cell therapy shows minimal toxicities in human CD147 transgenic mouse model with solid tumors.

ABSTRACT: The toxicity of chimeric antigen receptor-natural killer (CAR-NK) therapy has not been tested in solid tumors, compared with CAR-T therapy side by side. To address this, we investigated the CD147-CAR-NK "on-target/off-tumor" toxicity and neurotoxicity in human CD147-transgenic (hCD147TG) mice with hepatocellular carcinoma (HCC). We first tested the in vitro cytotoxicity of CD147-CAR-NK against CD147⁺ tumor and CD147⁺ healthy cells. Both CD147-CAR-NK cells and CD147-IL15-CAR-NK (autocrine expressing interleukin [IL]-15) can kill tumor cells specifically but not CD147⁺ healthy lung and spleen tissue from hCD147TG mice. In vivo assays show minimal systemic toxicities against CD147⁺ healthy tissues but 1-week-longer persistence times in

SUBMITTER: Sabha Y 

PROVIDER: S-EPMC11952776 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature