Inhibition of ERK1/2 mediated activation of Drp1 alleviates intervertebral disc degeneration via suppressing pyroptosis and apoptosis in nucleus pulposus cells.

ABSTRACT:

Objective

Dynamin-related protein 1 (Drp1) plays a crucial role in various inflammatory and degenerative diseases, yet its involvement in intervertebral disc degeneration (IVDD) remains poorly understood. This study aims to elucidate the mechanism by which Drp1 contributes to IVDD and to identify the efficacy of the Drp1 inhibitor Mdivi-1 on IVDD.

Methods

Tert-butyl hydroperoxide (TBHP) is utilized to induce an oxidative stress microenvironment in vitro. In vivo, IVDD model is constructed in 8-week old rats through puncture operation. The therapeutic effect of Mdivi-1 is evaluated through X-ray, MRI and histological analysis. A comprehensive set of experiments, including single-cell sequencing analysis, western blot, flow cytometry and immunofluorescence staini

SUBMITTER: Su S 

PROVIDER: S-EPMC11952795 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature