Comprehensive mapping of Arabidopsis alternative splicing landscape reveals key insights into plant development and immunity.

ABSTRACT: The different steps of alternative splicing (AS) in plants and its regulatory mechanisms have already been studied extensively. Its broader impact on cell identity, plant immunity-related genes, and their study as a whole remains to be investigated. Using transgenic plants, we sorted 11 different Arabidopsis thaliana cell types ranging from root to aerial organs using fluorescence-activated cell sorting. RNA-seq data were analyzed with vast-tools and enabled us to generate a high-resolution AS landscape across multiple cell types, all collected through the same experimental procedure. The analysis of cell type-specific gene expression and alternative splicing events highlights the importance of AS on transcription and AS regulation itself. AS is also shown to be tightly linked to cell iden

SUBMITTER: Barff T 

PROVIDER: S-EPMC11953613 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature