A human iPSC-Derived myelination model for investigating fetal brain injuries.

ABSTRACT: Cerebral white matter injuries, such as periventricular leukomalacia, are major contributors to neurodevelopmental impairments in preterm infants. Despite the clinical significance of these conditions, human-relevant models for studying fetal brain development and injury mechanisms remain limited. This study introduces a human iPSC-derived myelination model developed using a microfluidic device. The platform combines spinal cord-patterned neuronal and oligodendrocyte spheroids to recapitulate axon-glia interactions and myelination processes in vitro. The model successfully achieved axonal fascicle formation and compact myelin deposition, as validated by immunostaining and transmission electron microscopy. Functional calcium imaging confirmed neuronal activity within the system, unde

SUBMITTER: Hiraiwa T 

PROVIDER: S-EPMC11953958 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature