Genetic and epigenetic regulation of Treg cell fitness by autism-related chromatin remodeler CHD8.

ABSTRACT:

Background

Chromatin remodeler chromodomain helicase DNA-binding protein 8 (CHD8) defines a subtype of autism that is associated with immune disorders. It remains unknown whether CHD8 plays a cell-intrinsic role in immune cells such as regulatory T cells (Tregs) that maintain immune tolerance through suppressing CD4⁺ and CD8⁺ effector T cells.

Methods

Treg-specific conditional CHD8-deficient mice were generated by crossing Chd8^Flox/Flox mice with Foxp3^YFP-cre transgenic mice. Effects of CHD8 deficiency were investigated using hematoxylin and eosin (H&E) staining, flow cytometry, and multi-omics, including RNA-sequencing (RNA-seq), assay for transposase-accessible chromatin sequencing (ATAC-seq), and chromatin immunoprecipitation se

SUBMITTER: Yang JQ 

PROVIDER: S-EPMC11954365 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature