Heterologous expression and enzymatic properties of lipase from Mucor circinelloides.

ABSTRACT: Microbial lipases could be used to hydrolyze or recombine fats and oils, and had great applications in food processing, bioenergy and chemical industry. Mucor circinelloides was an important gamma-linolenic acid producing strain, and its genome was predicted to contain a large number of genes encoding lipases, the key enzymes in lipid metabolism. In the present study, a potential lipase WJ_23 from Mucor circinelloides WJ11 was cloned for the first time and heterologously expressed and purified to homogeneity in Pichia pastoris. By SDS-PAGE analysis, the molecular weight of the recombinant lipase was estimated to be ~ 34 kDa. The optimal temperature and pH of the recombinant lipase were 50 °C and 9.0, respectively. The recombinant lipase had good thermal stability at 50 °C with a broad pH s

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11954853 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature