Autologous glueless simple oral mucosal transplantation for the repair of limbal stem cell deficiency ocular surface in a rabbit model.

ABSTRACT: This study aims to investigate the efficacy of glueless simple oral mucosal transplantation to prevent the development of the limbal stem cell deficiency in rabbit model. Rabbit limbal stem cell deficiency (LSCD) models were constructed with alcohol and mechanical scraping. The oral mucosa and limbal tissue were harvested and cut into small pieces. Microincisions were made on the corneal limbus and the precut pieces were inserted in. Animals were divided to three groups: glueless simple oral mucosal epithelium transplantation (GSOMT), glueless simple limbal epithelium transplantation (GSLET) and LSCD group. Corneal epithelium defects, opacity and neovascularization were observed after surgery by slit-lamp microscopy. Neovascularization and epithelialization were scored. HE staining and imm

SUBMITTER: Zhang K 

PROVIDER: S-EPMC11954872 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature