Genetic targeting of myelinated primary afferent neurons using a new Nefh^CreERT2 knock-in mouse.

ABSTRACT: Primary afferent neurons that convey somatosensory modalities comprise two large, heterogeneous populations: small-diameter neurons that give rise to slowly conducting unmyelinated axonal C fibers and medium-to-large diameter neurons with fast myelinated A fibers. Despite these two major groupings, tools to differentiate between unmyelinated and myelinated primary afferent fibers by genetic targeting have not been available; in particular, whereas numerous mouse driver lines exist to target different C fiber populations, genetic tools that target myelinated primary afferent populations are scarce. Here we describe a knock-in mouse line expressing tamoxifen-dependent CreERT2 under control of the Nefh gene, which encodes neurofilament heavy chain (NFH or NF200), a protein that is highly enri

SUBMITTER: Chen JC 

PROVIDER: S-EPMC11954935 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature