RNA N<sup>6</sup>-methyladenosine demethylase FTO promotes diabetic wound healing through TRIB3-mediated autophagy in an m<sup>6</sup>A-YTHDF2-dependent manner.
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ABSTRACT: N6-methyladenosine (m6A) RNA modification impaired autophagy results in delayed diabetic wound healing. In this study, it was found that fat mass and obesity-associated protein (FTO) was significantly downregulated in the epidermis of diabetic patients, STZ-induced mice and db/db mice (type I and II diabetic mice) with prolonged hyperglycemia, as well as in different types of keratinocyte cell lines treated with short-term high glucose medium. The knockout of FTO affected the biological functions of keratinocytes, including enhanced apoptosis, inhibited autophagy, and delayed wound healing, producing consistent results with high-glucose medium treatment. High-throughput analysis revealed that tribbles pseudokinase 3 (TRIB3) served as the downstream target gene of FTO.
SUBMITTER: Dong Z
PROVIDER: S-EPMC11954964 | biostudies-literature | 2025 Mar
REPOSITORIES: biostudies-literature
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